Gene transfer in plants, agro bacterium mediated gene transfer. Crown gall disease, the tumour inducing principle and Ti plasmid, incorporation of T- DNA into plant cells
Indirect Gene Transfer :- When stable transformation is achieved by incorporating genes into the genome of plant cells with the help of a biological factor, it is called indirect gene transfer. Agrobacterium bacterium is used in this. Agrobacterium mediated Gene Transfer:- • Agrobacterium is a gram -ve bacterium found in soil around plant roots. • Two species of Agrobacterium bacteria cause diseases in dicot plants:- i. Agrobacterium tumefaciens ii. Agrobacterium rhizogenes i. Agrobacterium tumefaciens :- It causes Crown gall disease in angiosperm plants. Ti-plasmid is found in it. ii. Agrobacterium rhizogenes :- It cause hairy root disease in angiosperm plants. Ri - plasmid is found in it. • Recombinant DNA is made by incorporating the desired gene into the T - DNA portion of the Ti plasmid. Which are transferred to Agrobacterium cell. • Now cut round pieces of sterili...