Organ Culture:- Shoot Tip Culture or Meristem Culture
प्ररोह शिखाग्र संवर्धन या विभज्योतक संवर्धन (Shoot Tip Culture or Meristem Culture):-
· परिभाषा (Definition):-
जब निर्जमित दशाओं में प्ररोह के 0.1 से 1 mm शीर्ष भाग को पृथक करके कर्तोतक के रूप में उपयोग करते हुए संवर्धित किया जाता है तो इसे प्ररोह शिखाग्र संवर्धन कहते हैं। शीर्ष भाग में 0.05 से 0.1 mm विभज्योतक पाया जाता है, इसलिए इसे विभज्योतक संवर्धन भी कहते हैं।
(When in sterilized conditions 0.1 to 1 mm tip of the shoot is removed and cultured using it as an explant, it is called shoot tip culture. The 0.05 to 0.1 mm meristem is found in the apex, so it is also called meristem culture.)
· विभज्योतक संवर्धन वायरस मुक्त पौधे प्राप्त करने के लिए किया किया जाता है। विभज्योतक में कोशिकाओं का विभेदन नहीं होता है। संवहन ऊतक अनुपस्थित होता है। विभज्योतक कोशिकाओं में तीव्र उपापचय क्रिया होती है। वायरस एक कोशिका से दूसरी कोशिका में गति नहीं कर सकता।
(The meristem culture is performed to obtain virus-free plants. Differentiation of cells does not occur in the meristem. Vascular tissue is absent. Meristem cells have a rapid metabolic activity. The virus cannot move from one cell to another.)
· विधि (Procedure):-
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